AFLUID May 45/5

Brännström, Kristina, and William J. Arendshorst. Thromboxane A2 contributes to the enhanced tubuloglomerular feedback activity in young SHR. Am. J. Physiol. 276 (Renal Physiol. 45): F758–F766, 1999.—We performed micropuncture studies to determine the role of thromboxane A2 in the exaggerated tubuloglomerular feedback (TGF) activity in young spontaneously hypertensive rats (SHR). Glomerular function was assessed by changes in proximal tubular stopflow pressure (SFP) produced by different rates of orthograde perfusion through Henle’s loop. Seven-week-old SHR exhibited an exaggerated TGF activity compared with WistarKyoto rats (WKY) during euvolemia, confirming earlier studies. During control periods, the feedback-induced maximal SFP response (DSFP) was greater in SHR (18–19 vs. 12–13 mmHg in WKY), whereas basal SFP and proximal tubular free-flow pressure were similar in both strains. In one series, the thromboxane A2 agonist U-46619 was added to the tubular perfusate for a final concentration of 1026 M. In WKY, DSFP was increased by 100% to 26 mmHg. In contrast, DSFP in young SHR was unaffected by the thromboxane A2 agonist. In other animals, the thromboxane synthase inhibitor pirmagrel (50 mg/kg) was injected intravenously to inhibit thromboxane production. In SHR, pirmagrel decreased DSFP by 8.5 mmHg and reduced reactivity. Less attenuation was observed in WKY; DSFP was reduced by 3 mmHg, whereas reactivity was unchanged. In other studies, tubular perfusion with the thromboxane receptor inhibitor SQ-29548 (1026 M) reduced DSFP more in SHR (7 vs. 3 mmHg in WKY) and also decreased reactivity more in SHR (2.3 vs. 0.5 mmHg·nl21 · min21). Coperfusion of SQ-29548 and U-46619 resulted in an 85% block of the effect of U-46619 on DSFP. Tubular perfusion with the agonist U-46619 during thromboxane synthase inhibition markedly enhanced DSFP in both strains, with a greater effect in WKY. These results suggest that elevated levels of thromboxane A2 in young SHR contribute to the exaggerated TGF control of glomerular function in SHR during the developmental phase of hypertension.